The purpose of this study was to evaluate the effect of 20% Carbamide peroxide (CP), 10% Carbamide peroxide (CP) and 25% Hydrogen peroxide (HP) on surface roughness of enamel.\r\nMaterials and methods: Six young healthy dogs were used in this study. Sixteen teeth in each dog were selected (upper and lower incisors and canines) for bleaching. Group (1): Control, no bleaching treatment (upper & lower canines in each dog). Group (2): (1) & (2) dogs, bleaching of upper &lower incisors with 20% CP Opalescence. Group (3): (3) & (4) dogs, bleaching of upper &lower incisors with 10% CP Opalescence Group (4): (5) & (6) dogs, bleaching of upper &lower incisors with 25% HP Zoom2 N=24 Selected teeth on each dog were scaled and polished with a rubber cup, after that in groups 2,3,4 bleaching agents were applied for eight hours daily for two weeks. After sacrificed of the dogs, the teeth were stored in normal saline 0.9%. Enamel of labial surface of teeth was tested for surface roughness (Ra,um) using Environmental Scanning Electronic Microscope. Data were statistically analyzed using ANOVA followed by Duncan�s Multiple Range Test using SAS program.\r\nResults: No significant differences were found among the bleaching groups (2-3-4). However, all bleaching agents produced a significant increase in the mean surface roughness of enamel compared to the untreated control group (1) (p<0.05).\r\nConclusion: Different concentration of Carbamide peroxide and Hydrogen peroxide can promote alteration on enamel surface.
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